Izibonelo ze-Centrifugation

Ividiyo: Centrifugation| Separation Methods | Physics

-Delile

Izinhlobo ze-centrifugation
Izibonelo ze-centrifugation

I- i-centrifugation Kuyindlela yokwehlukanisa izinto eziqinile kusuka kuziphuzo zamandla ahlukahlukene enhlanganisweni, inqobo nje uma okwangaphambili kungancibiliki, kusetshenziswa amandla ojikelezayo noma amandla we-centrifugal.

Kulokhu, kuvame ukusetshenziswa ithuluzi elibizwa nge-centrifuge noma i-centrifuge, elizungeza ingxube ku-eksisi engaguquki nezimisele.

Njengoba igama layo liphakamisa (i-centrifuge: abalekela enkabeni), lo mbutho uvame ukusunduza izingxenye ezixineke kakhulu ngaphandle kwe-axis yokujikeleza, kushiye ezincane kakhulu enkabeni uqobo. Kuphambene namandla we-centripetal.

Bheka futhi: I-Chromatography

Izinhlobo ze-centrifugation

Ukwehluka. Ngokuya ngomehluko wobuningi bezinto, kuyindlela eyisisekelo kepha engafani.
Isopychnic. Le ndlela isetshenziswa, ngokwesibonelo, ukwahlukanisa izinhlayiya ezinosayizi ofanayo kepha ngobukhulu obuhlukile.
Indawo. Umehluko kusilinganiso sedimentation sezinto (ngenxa yobuningi bazo obuhlukile) sisetshenziselwa ukuhlukanisa ngesikhathi esithile se-centrifugation.
Ultracentrifugation. Amandla ayo avumela ukuhlukaniswa kwama-molecule nezinto ezingaphansi kwamaseli.

Umshini wokuwasha. Lo mshini usebenzisa amandla we-centrifugal ukuhlukanisa izingubo (eziqinile) emanzini (ketshezi) ngokuya ngobuningi bazo. Yingakho izingubo zivame ukoma lapho zisuswa ngaphakathi.
Imboni yezobisi. Ubisi luhlukaniswe ngamasentimitha ukuhlukanisa amanzi kanye nokuqukethwe kwe-lipid, ngoba okusetshenziselwa lokhu kusetshenziselwa ukwenza ibhotela, noma ubisi olunamakhemikhali kusuka okusele.
Izimoto ejikeni. Lapho sishayela ngejubane emgwaqeni osemgwaqweni, sivame ukuzwa amandla asidonsela ngaphandle komgwaqo, kude ne-eksisi egobile. Lokho kungamandla we-centrifugal.
Ukuthola ama-enzyme. Emkhakheni wezokwelapha nowezidakamizwa, i-centrifugation ivame ukusetshenziselwa ukuthola ama-enzyme athile kumaseli akhethekile awakhiqizayo.
Ukuhlukaniswa kwe-DNA. I-Isopycnic centrifugation ivame ukusetshenziselwa kumalabhorathri wezakhi zofuzo ukuhlukanisa i-DNA yeselula futhi ivumele ukuqhubeka nokuqhutshwa kwayo.
Ukudla kwama-celiacs. Uma kukhulunywa ngokuhlukanisa amaprotheni kusuka ku-gluten ekudleni okukuwo, inqubo ye-centrifugation ibalulekile. Kwenziwa ekunamathiseleni kwesitashi, okuqukethwe kwe-gluten kufinyelela ku-8%, futhi kwehliselwa ngaphansi kuka-2% kuma-centrifugations akhethiwe alandelanayo.
Ukuhlolwa kwegazi Kusetshenziswa i-centrifuge ukwahlukanisa izakhi zegazi, njenge-plasma nezinye izinto ezivame ukuxubana kulo.
Ukusheshisa kwe-sedimentation. Ezimbonini ezahlukahlukene zokudla, njengokuphisa noma okusanhlamvu, i-centrifugation isheshisa izinqubo zokwenziwa kwe-sediment ezikhiqizwa amandla adonsela phansi okuzenzekelayo, kunciphisa isikhathi sokulinda kwempahla eluhlaza.
Ukuhlanza i-latex. Emkhakheni we-latex, kuyadingeka ukuhlanza into, engaphezulu kwayo ethambekele kakhulu ekunamatheleni kwezinye izinhlayiya, futhi lokhu kwenziwa nge-centrifugation, unikezwe ubukhulu obuphansi bezinto.
Ukomiswa kokuqina. Okunye ukusetshenziswa kwezimboni kwe-centrifuge ukumiswa kwamakristalu noma ezinye izinto umkhiqizo wazo ohambisana namanzi. Lapho ejikeleza, amanzi ayahlukana nokuqina futhi alahlwe, kusale okuqinile okufunwayo ngaphandle ketshezi.
Ukuphathwa kwendle. Ukufakwa phakathi kwamanzi angcolisiwe kuvumela ukukhishwa kwezinto eziminyene ngaphakathi, hhayi kuphela ukudla okuqinile, kepha ngisho namafutha, amafutha nezinye izinto ezingafuneki ezingalahlwa.
Amapaki wokuzijabulisa. Abagibeli abaningi bepaki lokuzijabulisa basebenzisa amandla we-centrifugal ukwenza umphumela wabagibeli kubagibeli babo, abasheshe bazungeziswe ku-eksisi engaguquki, baboshwe ngokuqinile esihlalweni esivimbela ukuba baphonswe ngaphandle kwe-axis yokujikeleza.
Abagibeli bezithuthuthu iPirouette. Umshayeli wesithuthuthu emkhakheni uyisekisi esezingeni eliphakeme, okwazi ukushayela ngophahla lomkhakha odelela amandla adonsela phansi. Lokhu kuyakwazi ukukwenza ngemuva kokwenza ukujika okuningi ku-eksisi efanayo evundlile, ukunqwabelana kwejubane nokuthumela kumandla we-centrifugal ayinamathela ingaphakathi lomkhakha. Ekugcineni lo mbutho uzokuba mkhulu kakhulu ukuthi uzokwazi ukulinganisa ukunyakaza nokuphikisa amandla adonsela phansi.
Ukuthambekela kwamathrekhi esitimela. Ukumelana namandla we-centrifugal, ujantshi wesitimela uvame ukugoqelwa emajikeni, usebenze ukumelana ukuze unganqotshwa amandla awusunduzela ngaphandle futhi ungaphambuki.
Ukuhumusha kwasemhlabeni. Isizathu sokuthi amandla adonsela phansi e-Sun angasicindezeli singene kuwo kungenxa yamandla we-centrifugal okuthi, lapho ejikeleza ekseni lenkosi yelanga, awusunduzele ngaphandle, ulwe futhi ulinganise ukukhanga kwamandla adonsela phansi.